Identification of S, F1C and three PapG fimbrial adhesins in uropathogenicEscherichia coliby polymerase chain reaction

Application of specific primers and polymerase chain reaction for identification of ralstonia solanacearum biovar2

Development of New Modified Simple Polymerase Chain Reaction and Real-time Polymerase Chain Reaction for the Identification of Iranian Brucella abortus Strains

Brucellosis is primarily a worldwide zoonotic disease caused by Brucella species. The genus Brucella contains highly infectious species that are classified as biological threat agents. In this regard, the identification of Brucella can be a time-consuming and labor-intensive process posing a real risk of laboratory-acquired infection to the laboratory staff. This stud...

Polymerase chain reaction assay targeting nox gene for rapid identification of Brachyspira canis in dogs

Genus Brachyspira,as Gram negative anaerobic bacteria, colonize in dogs intestine. The aim of the current study was to determine the prevalence of Brachyspira spp. for the first time in Iran and rapid identification of Brachyspira spp. in dogs by a new designment of a species-specific primer set for B. canis. One hundred fifty-one fecal samples were obtained ...

Application of culture and polymerase chain reaction (PCR) methods for isolation and identification of Mycoplasma synoviae on broiler chicken farms

Mycoplasma synoviae (M. synoviae) is a major worldwide poultry pathogen that causes serious economic losses in the poultry industry. This study was designed to detect M. synoviae through culture isolation and polymerase chain reaction (PCR) assay to demonstrated the involvement of M. synoviae infection in trachea and the lung/air sac samples taken from commercial broiler chicken farms in 3 m...

Isolation and identification of Mycoplasma agalactiae by culture and polymerase chain reaction (PCR) from sheep of Qom province, Iran

Contagious agalactia (C.A) is an infectious syndrome of sheep that is characterized by mastitis and subsequent failure of milk production, arthritis, abortion and keratoconjunctivitis. Mycoplasma agalactiae (M. agalactiae) is the main cause of the disease in sheep. The aim of this study was isolation and identification of M. agalactiae with culture and polymerase chain reaction (PCR) assay from...